The PCR Thermal Cycler is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). The PCR Thermal Cycler raises and lowers the temperature of the samples in a holding block in discrete, pre-programmed steps, allowing for the denaturation and reannealing of samples with various reagents. Thermal cyclers may also be used in laboratories to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid diagnostics.
In recent years, with the rapid development of molecular biology technology, research and application in related fields have achieved more results. Among them, the birth and evolution of PCR technology are undoubtedly a more important part. The instrument that assists this key technical means is the most common gene amplification instrument in our laboratory. PCR technology is a molecular biology technique used to magnify and amplify specific DNA fragments. It can be regarded as a special DNA replication in vitro. What is Commonly used in the market at present are ordinary PCR instruments, gradient PCR instruments, in situ PCR instruments, fluorescence quantitative PCR instruments (qPCR), digital PCR instruments (dPCR), etc. The most used PCR Thermal Cyclers are gradient PCR machines and fluorescent quantitative PCR machines.
PCR Thermal Cycler is used to amplify a DNA fragment located between two known sequences, similar to the replication process of natural DNA. The DNA molecule to be amplified is used as a template, and a pair of oligonucleotide fragments complementary to the 5'end and 3'end of the template are used as primers. Under the action of DNA polymerase, it follows the template according to the half-reserved replication mechanism. Chain extension until the completion of new DNA synthesis, repeat this process, and the target DNA fragment can be amplified.