English
A laboratory centrifuge is a piece of laboratory equipment, driven by a motor, which spins liquid samples at high speed. It is an indispensable tool for separating serum, precipitating tangible cells, concentrating bacteria, PCR tests, liquid-solid separation, and so on. There are various types of centrifuges, depending on the size and the sample capacity.
Heal Force lab centrifuge is divided into High-speed refrigerated centrifuges and non-refrigerated centrifuges.
Lab centrifuge is a machine that uses centrifugal force to separate the contents of a sample based on its density. A centrifuge works by using the principle of sedimentation: Under the influence of gravitational force, substances separate according to their density.
1. Rotating Speed:
Each lab centrifuge has a rated maximum speed. The maximum speed refers to the speed under no-load conditions, but the maximum speed depends on the type of rotor. There are differences depending on the size of the sample quality. The maximum speed is different depending on the rotor; an imported centrifuge can be equipped with multiple rotors, The horizontal rotor can reach 15000rpm/min, but the angular rotor can reach about 14000rpm/min. Therefore, you must be cautious in the choice of speed. The maximum centrifuge you choose The speed is higher than the target speed.
For example, the target speed is 16000rpm/min, and the maximum speed of the selected centrifuge must be higher than 16000rpm/min.
Generally, the separation effect is mainly determined not by the rotation speed, but by the centrifugal force, so sometimes the rotation speed does not meet the requirements, as long as the centrifugal force can reach the standard, it is the same, and the experiment can achieve the effect you need.
Centrifugal force calculation formula: RCF=11.2×R×(r/min/1000) 2R represents the centrifugal radius, and r/min represents the rotation speed.
2. Temperature:
Some samples, such as proteins, cells, etc., will be destroyed in a high-temperature environment, so you must choose a refrigerated centrifuge, which has a rated temperature range. When the centrifuge is running at high speed, the heat generated by the centrifuge is balanced with the refrigeration system of the centrifuge at a certain temperature. Generally, samples for refrigerated centrifugation need to be kept at 3°C to 8°C. The specific amount depends on the rotor, such as the rated temperature of a centrifuge. The range is -10℃～60℃, and it can reach about 3℃ when rotating with a horizontal rotor. If it is an angle rotor, it may only reach about 7℃.
3. Volume:
How many sample tubes need to be centrifuged each time? How much capacity does each sample tube need?
These factors determine the total capacity of a centrifuge. Simply put, the total capacity of the centrifuge = the capacity of each centrifuge tube × the number of centrifuge tubes. The total capacity and the workload are matched.
4. Rotor:
Laboratory centrifuge rotors are mainly divided into two types, horizontal rotors: the hanging basket is in a horizontal state during operation, at right angles to the rotating shaft, and the sample concentrates the sediment on the bottom of the centrifuge tube; angle rotor: the centrifuge container and the rotating shaft form a fixed angle, and the sample will settle Focus on the bottom of the centrifuge tube and the side wall near the bottom. If you want the separated samples to concentrate on the bottom of the centrifuge tube, choose a horizontal rotor. If you want the sample to concentrate on the bottom of the centrifuge tube and on the side wall near the bottom, choose an angular rotor.
There are also some special tests or special samples that require special rotors such as large-capacity hanging baskets are mostly used in blood stations, microplate rotors, slide rotors, PCR rotors, test tube rack rotors, and capillary rotors. The rotor has a fixed specification, which is combined with the capacity of the centrifuge, such as a 36×5ml angular rotor, which determines the type of the rotor and the capacity of the centrifuge, so the choice of the rotor is very important.
5. Control System:
High-end centrifuges all use microcomputer control systems, which can not only ensure the safe operation of the centrifuge but also complete the tasks automatically. Many centrifuges now have better-humanized control systems. For example rotor identification function, safety lock function, fault prompt function, acceleration and deceleration curve, and so on. Refrigerated centrifuges are also different in terms of refrigeration. The current environmentally friendly technology is of course CFC-free refrigeration.
In addition, the noise problem must be considered, and try to choose a centrifuge with less noise, so as to maintain a comfortable experimental environment. Be cautious in terms of accessories. Some experiments need to use special centrifuge tubes to centrifuge toxic samples or samples that require ultra-high-speed centrifugation. Such centrifuge tubes must be equipped with corresponding tube sleeves to be safer. There are also some special sample containers, irregular sample bottles, blood bags, etc. These details and accessories must be carefully considered when choosing a centrifuge, otherwise, normal work cannot be performed.
How to ensure the safe operation of the lab centrifuge and give full play to its performance is a major concern of technical and experimental personnel. In order to improve the utilization rate and extend the service life of the instrument, ensure the safe operation of the instrument, and avoid failures, we have compiled the following guidelines for operating Heal Force laboratory centrifuge safely and related precautions.
1. Turn on the machine and press the door key to open the centrifuge door.
2. Select the appropriate rotor and adapter.
3. Clean the rotor, centrifugal cavity, and adapter.
4. Install the rotor and adapter.
Notes:
1. Hold the rotor with both hands and align the center hole of the rotor with the locking sleeve.
2. Put it down vertically and put it on the bottom of the cone.
3. Let go of your hands, and then lightly press the rotor with your hands to confirm that the bottom of the rotor has been placed at the designated position.
4. Using the included Allen key, tighten the lock screw in a clockwise direction.
Improper operation of the laboratory centrifuge will often cause more serious consequences. During the experiment, we must pay special attention to the operating specifications to ensure the correct use of the centrifuge. First-time users should consult personnel with operating experience or refer to the manual. The equipment should be used with care, not blindly.
1. Load the centrifugal sample in the correct position.
2. Tighten the rotor cover, and slowly close the centrifuge door cover with both hands.
3. Set the parameters and start the centrifugal experiment.
4. After centrifugation, press the door button, open the door cover gently with your hand, and take out the sample.
Notes:
1. Before the rotor is running, the samples and test tubes should be loaded accurately and symmetrically (need to be balanced). The centrifuge tube can be loaded with samples at different times, but the samples must be loaded symmetrically. The asymmetric loading sample chamber must never be turned on.
2. When the separation sample you are using is contaminated or to prevent the separation sample from being contaminated, remove the sample and rotor from the shaft together and put it in the biological safety cabinet before performing other operations.
3. When closing or opening the door of the centrifuge, hold the door with your hand and operate it slowly and gently.
At the end of the centrifugal experiment every day, take out the rotor and wipe the centrifuge chamber with a clean cloth. Wiping is mainly to remove dust, condensed water, debris, and leftovers of previously separated samples in the centrifuge chamber, and confirm that the chamber is clean and tidy. Steps to take out the rotor:
1. Using a special wrench to remove the rotor, and loosen the locking screw in a counterclockwise direction (no need to take it out).
2. Grasp the rotor with both hands, lift it up vertically and don't shake it from side to side.
3. If you are using a horizontal rotor, first take out the centrifuge container (such as the centrifuge hanging cup).
