Outbreak background

The 2019 Novel Coronavirus, or “2019-nCoV“, was discovered because of Wuhan Viral Pneumonia cases in 2019, and was named by the World Health Organization on January 12, 2020.

According to Zhong Nanshan, who is the Academician of Chinese Academy of Engineering, the epidemic was concentrated in two districts of Wuhan, both of which have relatively large seafood markets. Preliminary epidemiological analysis shows that there is a high possibility of transmission to humans through wild animals.

Source of 2019 Novel Coronavirus

At present, it has been confirmed that some people and medical staff are infected. Therefore, all laboratories are requested to pay special attention to biosafety protection, and in particular, a biosafety cabinet must be selected for protection against 2019 Novel Coronavirus. Here is the live story of biosafety cabinets installed to fight against the virus.

biosafety cabinets to protect medical staff from 2019 Novel Coronavirus

Symptoms of infection

Early general symptoms of 2019 Novel Coronavirus: 1. Fever, mainly manifested as a fever similar to a common cold, etc .; 2. Weakness, feeling weak and weak in the limbs; 3. Dry cough; 4. Feeling breathing becomes increasingly difficult, which is also The biggest difference from the common cold.

In the later stages, it is manifested as acute respiratory distress syndrome. If it is prolonged or very severe, it is manifested as septic shock. One of the most important manifestations is difficult to correct metabolic acidosis. This kind of performance cannot be obtained through daily observation. The last major medical manifestation is coagulopathy.

Clinical diagnosis of 2019 Novel Coronavirus

Current routine detection ways

  • Serological detection: detection of serum-specific antibodies, such as ELISA, immunofluorescence and other methods; neutralizing antibodies: detection using plaque reduction neutralization test methods;
  • Pathogenic detection: A: Nucleic acid detection: Fluorescence quantitative RT-PCR method; B: Virus isolation: The specimen is separated and cultured. After the lesion appears, the virus is identified by the method of detecting nucleic acid.

Advantages of quantitative PCR

  • High sensitivity: can detect single cell genes
  • High specificity and accuracy
  • Simple and fast operation: through calculation, integration of PCR amplification, product detection and quantitative analysis
  • Safe and pollution-free, perform PCR amplification and product analysis in a fully enclosed state
  • The result observation has good reproducibility, and the real-time observation online, the result judgment is intuitive, avoiding human factors interference, especially suitable for early detection

Since the whole genome of the Wuhan coronavirus, 2019-nCoV, sequenced on January, 2020, various in vitro diagnostic companies can use this as a basis to develop nucleic acid detection kits. The public indicates that the corresponding detection kit has been successfully developed, and the above products are only used for the detection of 2019 Novel coronaviruses and other coronaviruses.

Labortory testing guidance by WHO

According to the “Laboratory testing for 2019 novel coronavirus (2019-nCoV) in suspected human cases” issued by WHO, the basic process of PCR testing for new coronavirus is as follows:

Specimen collection

Collect both upper respiratory tract (URT, including nasopharynx and oropharynx) and lower respiratory tract (LRT, including sputum, tracheal aspirate or bronchoalveolar lavage fluid), Detect the nCoV by RT-PCR. Where lower respiratory tract samples are readily available (eg mechanically ventilated patients, the clinician may choose to collect only lower respiratory tract samples): Use appropriate PPE for specimen collection (note droplet and contact precautions for URT specimens and air for LRT specimens Precaution). When collecting URT samples, use virus sampling cotton swabs (sterile polyester or rayon, not cotton) and virus transport media. Do not take samples from nostrils or tonsils.

For patients suspected of having nCoV, especially those with pneumonia or severe disease, a single URT sample cannot rule out a diagnosis, and it is recommended to increase URT and LRT samples. LRT (relative to URT) samples are more likely to be positive, and as described in 4.1, clinicians may choose to collect only lower respiratory tract samples in cases where lower respiratory tract samples are readily available (eg, patients with mechanical ventilation).

Nucleic acid extraction

Equipment and consumables for nucleic acid extraction of 2019 Novel Coronavirus:
Biological safety cabinet (function: sample separation, providing personnel, samples and environmental protection), desktop high-speed refrigerated centrifuge (function: speed ≥ 12000RPM, analysis of nucleic acids, etc.), low temperature refrigerator (function: reagent storage), metal bath, pipette (Function: accurate loading), sterilized centrifuge tube (function: separation of nucleic acids, etc.), latex gloves (function: anti-pollution), anhydrous ethanol, isopropanol, DNase and RNase-free ultrapure water (non-DEPC treated water).

Here are some tips when choosing the relevant equipment

Note when purchasing biological safety cabinets: Since it is direct personnel safety protection equipment, it is recommended to purchase reliable brand’s products, such as HealForce’s HFsafe-1200LC, The operating area cleanliness level is higher, such as HFsafe-12CY, KSP series.

Note when purchasing fully-automatic nucleic acid extraction: Choose the the right number of channel based on the sample size and focus on magnetic bead extraction technology.

Caution when purchasing high-speed refrigerated centrifuges: choose refrigerated, biosafety-protected rotors, such as Neofuge13R.

Note when purchasing ultrapure water system: choose reliable ultrafiltration, the water capacity can be 10-15L, such as Smart Plus NT series

Real-time PCR detection

Euipment and consumables: Biological safety cabinet (function: sample preparation, providing personnel, samples and environmental protection), real-time PCR instrument (function: PCR amplification and detection analysis), desktop high-speed refrigerated centrifuge (function: speed ≥12000RPM), low temperature refrigerator (- 86 ℃ sample storage, -20 ℃ and 4 ℃ reagent storage), shaker, pipette (function: accurate loading), sterilized centrifuge tube (0.5 / 1.5 / 2ml), 0.2ml optical grade PCR tube / plate ( function: PCR reaction tube), latex gloves (function: anti-pollution).

Reagent & Kits: It is recommended that reagents from listed professional reagent companies such as BGI, Daan Gene, Wondfo, should be selected for clinical use for detection of 2019 Novel Coronavirus. Nucleic acid extraction, reaction system configuration, and interpretation of results are based on the kit instructions.


Lab equipment for PCR laboratories setup

Contact Heal Force team for more information and technical assistance in selecting the right equipment for your PCR laboratory.


EuipmentModelBrand
Shaker and mixerQL-901Jinagsu
Dry bathK30Hangzhou
Biosafety cabinetHFsafe-1200LCHeal Force
High speed centrifugeNeofuge 13RHeal Force
Ultrapure water systemSmart Plus N VFHeal Force
Pharmacy refrigeratorMPC-5V310Heal Force
Deep freezerMDF-25V260Heal Force
Ultra low temp freezerMDF-86V400Heal Force
Clean benchAplphClean1300Heal Force
Automated Extraction
Instruments
Smart LabAssist-32 Advenced
Nanotech Inc
Thermal cyclerT960Heal Force
Real-time PCRX960Heal Force
Pipette 0.5-10ulEppendorf
Pipette 5-50ul Eppendorf
Pipette 20-200ul Eppendorf
Main lab equipment and kits for PCR clinical laboratorys


Automated extraction system

This system is an automatic nucleic acid extraction instrument produced by Advenced Nanotech Inc. The Smart LabAssist-32 instrument is automated, fast and simple. A 96-well plate is used to preload the reaction plate, and 1-32 samples can be extracted at the same time. With different kinds of magnetic bead nucleic acid extraction reagents, it can extract serum, blood, body fluids, animal and plant tissues and other types of specimens.

▲ Maximum sample volume: 1000ul
▲ Maximum number of samples: 32 tests / per batch
▲ Operating time: 30-50min / per batch
▲ Program editing, U disk import and export function
▲ Color full touch screen operation
▲ With adjustable heating function
▲ UV light sterilization function that can be switched on and off regularly

Biological safety cabinet

The product has obtained CFDA, NSF49, EN12469-GS certifications for three different international standards. It is the first brand that has obtained these three certifications at the same time in Asia. The company is one of the main developers and amendments of China’s biosafety cabinet standards.

▲ AAF / Camfil ULPA ultra-high efficiency air filter, patented filter bar code display alarm technology.
▲ German original EBM brand self-induction variable frequency fan, linked with the micro airflow sensor
▲ Adopt thermal micro airflow speed sensor to monitor in real time, multi-point measurement, with energy-saving mode;
▲ Perform KI-Discuss personnel protection test
▲ Aboudant experience in the design of automatic linkage control of laboratory exhuast system.

Vertical laminar flow clean bench

AlphaClean clean benches are ideal for a wide range of appilcations where sample protecton is required. Outstanding safety is ensured through a variety of core components and features to improve cleanliness

▲ Dual monitoring sensor to control airflow
▲ Barcode filter life span monitoring
▲ Standard design of door lock security for easy equipment management
▲ Multi-parameter display with color screen, with UV lamp reservation function

T960 gradient thermal cycler

For over 20 years, we have specialized in thermal cylers with proven performance and unmatched combination of features, just right for every laboratory, whether you run only a few samples per week or perform high-throughput processes.

▲ 7-inch color touch screen operation
▲ Imported TEs with long service life
▲ Multiple blocks are replaced easily, and gold-plated modules are equipped as standard.
▲ High degree of intelligent network and remote control

Neofuge 13R tabletop high speed centrifuge

This high speed laboratory centrifuge is marked and approved by CE and ISO9001, ISO13485. This model provides excellent solution for your sample preparation in PCR labs.

▲ Maximum speed ≥15000 rpm, maximum centrifugal force ≥22000 * g, maximum capacity 16 * 5ml
▲ Knob type parameter setting, multi-parameter display on color screen;
▲ Airtight sterilizable biological safety protection rotor, which can automatically identify the rotor;
▲ With fast pre-cooling function, the time from room temperature to 4 ℃ <6 minutes

Smart Series water purification system

PCR laboratories require ultrapure/pure water every day. We understand how important it is for scientists and medical staff to obtain ultrapure water for most critical science and analytical applications.

▲ The whole machine is made of plastic molds, which can easily and conveniently replace consumables;
▲ Patented multifunctional water tank to ensure stable water quality;
▲ Equipped with a remote water dispenser;
▲ Use high quality consumables such as RO film, resin, etc. to ensure water quality;
▲ With alarm function of consumable failure;

Utra low temperature freezer

Product range: Pharmacy refrigerator, -25/-40℃ deep freezer, -86℃ ultra low temperature freezer

▲ Dual cascade technology, optional dual system
▲ Second-generation VIP technology, good insulation effect
▲ 380L volume noise as low as 50 decibels
▲ The external heat exchanger is externally installed for easy maintenance
▲ Complete alarm function, worry-free use

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