Use of BSC

As a first-level barrier in the biosafety laboratory, the biological safety cabinet is indispensable hardware equipment in the current anti-pandemic research laboratory. A cytotoxic safety cabinet is also a necessity if the biological experiment involves cytotoxic drugs handling. 

The Class II biological safety cabinet has a front window operation port through which the personnel conduct the operation. The negative pressure air flow drawn inward from the front window operation port is used to protect the safety of the operator; The downward airflow is used to protect the experimental items in the biosafety cabinet; the airflow is discharged through a high-efficiency filter.

Biological Safety Cabinet

Type A2 (air flow): 70 % Recirculation 30 % Exhaust

Type B2 (air flow): 100 % Exhaust

On the basis of technical experience for decades, we have summarized the following precautions referring to customer feedback and common problems. We hope that laboratory personnel could study carefully and avoid risks as much as possible in actual operation.

Use of Biological Safety Cabinets

1 Environmental requirement: indoor use, avoid direct sunlight, 10-30 ℃ ambient temperature;the wind speed must be re-corrected at high altitudes.

2 The equipment should be installed on an immovable horizontal surface.

3 The equipment must be installed close to a fixed power outlet. Under the circumstances of no external drainage system, the top is at least 200mm away from the top of the room or the top obstacle, at least 200mm away from the wall on both sides, and at least 300mm away from the wall to facilitate smooth exhaust air flow.

The positions A, D and E in the picture are the most suitable for the installation of biological safety cabinets. In order to prevent interference, the equipment should not be installed in the passageway where people come and go. Do not make the BSC sliding front window too close to the laboratory doors or windows. Besides, the room needs to be clean.

Operation Notes of BSC

1 Once the filter end-of-life prompt appears, it is recommended that you stop using the machine and detect whether there are other abnormalities, and notify the professional engineers of the manufacturer to test the filter integrity and air flow rate of the device. In case this requirement can not be satisfied, inform the relevant personnel immediately to replace the air filter.

2 If any items need to be taken out or moved in during operation, please move your arm as slowly as possible to reduce air disturbance and avoid the escape of pollution caused by the disturbance of the airflow layer.

3 Place the items needed for the work as far as possible in front of you to avoid twisting your neck frequently.

4 Place items reasonably in the working area to reduce your operating distance.

5 Use your entire shoulders and arms instead of just your wrists while moving items.

6 Take a break of 5-10 minutes after working continuously for more than 2 hours. By doing radio gymnastics, you can stretch and adjust your limbs and reduce fatigue. Eye exercises are recommended to protect eyes and eyesight.

7 Personal protection requirements: Wear PPE clothing, masks, cap and gloves when operating biological safety cabinets. Wear protective goggles or face mask as needed.

Biological Safety Cabinet

8 Principles for placing items in biological safety cabinets

Open flames can cause the airflow of the biological safety cabinet to be turbulent. Interfering with the airflow pattern could damage the ULPA air filter, causing danger when handling volatile and flammable materials. The use of alcohol lamps should be prohibited, use infrared sterilizers instead.

Routine Maintenance of BSC

Normally, we have to clean the countertop of the biosafety cabinet to avoid residues of dirt after the experiment. If the contamination of the operating surface of the biological safety cabinet is not cleaned up in time, we can use 75% alcohol and gauze for cleaning.

After years of experience by experimenters, it is not recommended to use absorbent cotton products to wipe the tabletop, because it will cause fine cotton wool to hang on the tabletop, easy to breed bacteria. Cleaning agents containing chlorine is also not recommended that will cause certain corrosion of stainless steel countertops.

If an alarm occurs in the biosafety cabinet, contact a professional maintenance engineer for the first time who would provide on-site guidance to perform equipment maintenance.

In view of the expiration of the filter’s service life, professional engineers are required to conduct fumigation and disinfection and then dismantle and replace it. The replaced filter should be treated as experimental waste and not treated as domestic waste.

Suggestions

In the course of your experiment, there will always be various bacteria and viruses threatening your health and life safety therefore you have to choose a biological safety cabinet and take protective measures.

HFsafe Series Biological Safety Cabinet

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