Immature test kits: The kits were launched urgently at the beginning of the epidemic, so the early kits are Immature, and some products only use two conserved sequences even one conserved sequence, which is less accuracy compared to three conserved sequences.
Irregular sampling:Common errors include incorrect collection location and insufficient sample collection. what’s more, at different stages of infection, the collection location will also change. For example, if virus infects the trachea, it is difficult to collect the virus in the throat.
Irregular operation methods: Large-scale screening leads to the need for operators to test a large number of samples every day. Inadvertent operation between samples is prone to sample contamination, resulting in inaccurate data (but the latest batch of kits equip UNG enzyme and dUTP can effectively reduce the contamination between samples)