Introduction

Clinical Gene Amplification Testing Laboratory, also known as Molecular Laboratory and PCR Laboratory, has gradually become an indispensable part of the inspection field. In principle, clinical gene amplification testing laboratory should set up the following areas: Reagent Storage and Preparation Area, Specimen Preparation Area, Amplification Reaction Area, Amplification Product Analysis Area.

The four areas must be completely independent of each other in physical space. Whether in space or in use, each area should always be in a completely separated state, with no direct air communication.

Because of the different functions of the various areas, the commonly used instruments are also different. Let’s dive deeper into what kind of equipment should be equipped with the PCR laboratory design and the basis of different experimental partitions.

PCR Laboratory

PCR laboratory is also called gene amplification laboratory. PCR is short for polymerase chain reaction. It is a molecular biology technique used to amplify specific DNA fragments, regarded as special DNA replication in vitro. Through the DNA gene tracking system, you can quickly grasp the virus content in the patient’s body. Its accuracy is as high as nanometers, precisely detecting the amount of hepatitis B virus in the patient’s body, whether it is replicated or infectious, how strong the infectivity, whether it is necessary to take medicine, and whether there is an abnormal change in liver function, conductive to timely determine which type of antiviral drug is most suitable for the patient, judge the efficacy of drugs, and provide a reliable test basis for clinical treatment.

PCR Laboratory Graphic Design Layout

In principle, the PCR amplification laboratory is divided into four separate working areas: reagent storage and preparation area, specimen preparation area, amplification reaction area, and amplification product analysis area. In order to avoid cross-contamination, entering each work area must strictly follow a single direction, that is, only from the reagent storage and preparation area → specimen preparation area → amplification reaction area → amplification product analysis area. The transfer of reagents and samples between the experimental areas should be carried out through the transfer window.

(1) In the physical space, they must be completely independent of each other. Whether the area is idle or in use, it should always be completely separated;
(2) No direct air communication.

Idealized PCR Laboratory Design

PCR Laboratory

Illustration of PCR laboratory area

Reagent Storage and Preparation Area (PCR AreaⅠ)

Refrigerator (2-8 ℃)Refrigerator ( below -20 ℃)Vortex Oscillator
Removable UV lampMicropipettePCR reaction tube
PCR reaction tube rackDedicated lab coatDisposable powder-free gloves
Pipette tipOffice supplies

Function: preparation of storage reagents, preparation of reagents and amplification reaction mixture, and storage and preparation of consumables such as centrifuge tubes and pipette tips.

Specimen Preparation Area (PCR Area Ⅱ)

Refrigerator (2-8 ℃)Refrigerator ( below -20 ℃)Vortex Oscillator
Removable UV lampMicropipetteHigh-speed centrifuge
Biosafety CabinetMetal bathMini centrifuge
Nucleic acid extractor (Optional)Disposable powder-free glovesPipette tip
Dedicated lab coat, office suppliesFully automatic nucleic acid extraction and system construction workstation

Function: nucleic acid (RNA, DNA) extraction, storage and addition to the amplification reaction tube. The operations involving clinical samples should comply with the requirements of BSL-2 protective equipment, personal protection and operating specifications.

Amplification Reaction Area (PCR Area Ⅲ)

Removable UV lampMicropipetteReal-Time PCR Detection System
Disposable powder-free glovesPipette tipDedicated lab coat, office supplies

Function: DNA amplification and detection.

Amplification Product Analysis Area (PCR Area Ⅳ)

Molecular hybridization instrumentElectrophoresisSequencer
Removable UV lampMicropipetteDedicated lab coat
Pipette tipOffice supplies

Function: Further analysis and determination of amplified fragments, such as hybridization, digestion electrophoresis, denaturing high-performance liquid analysis, sequencing, etc. (Different projects correspond to different instruments)

Heal Force PCR Laboratory Solution

We aim to provide you with an integrated solution for laboratory equipment and instruments regarding novel coronavirus nucleic acid detection.

PCR laboratory Decoration

Laboratory walls, ceilings and floors should be smooth, easy to clean, leak-proof and resistant to chemicals and disinfectants. The ground should be non-slip, and carpets and curtains must not be laid in the laboratory. The ceiling should have a solid structure, good airtightness, and all the negative corners adopt a circular arc line transition; the inner wall of the wall is smooth, non-adsorption, corrosion-resistant, easy to clean and disinfect; the wall and ceiling are recommended to use color steel plate.

The ground material should meet the requirements of seamless, no leakage, smooth and corrosion-resistant.

Choose the lamps and lanterns with the function of purification that can achieve the characteristics of easy cleaning and no dust accumulation. The laboratory should be equipped with emergency lighting devices. Also, consider the appropriate installation location to ensure that personnel leave the laboratory safely.

Reliable power supply and emergency lighting. Each room must be equipped with ultraviolet germicidal lamps, the installation height of germicidal lamps is between 1.8 ~ 2.1m.

An ultraviolet lamp is installed inside the transfer window for disinfection is required.

It should be equipped with enough fixed power sockets to avoid using multiple devices with a common power socket. There should be a reliable grounding system where leakage protection devices or monitoring and alarm devices should be installed at key locations.

The laboratory table cabinets and chairs should be stable and firm, with round corners. The benchtop should be waterproof and resistant to heat, organic solvents, acids and alkalis, disinfectants and other chemical agents. Epoxy countertops are generally used.

The cabinets and test benches in the test bench should be firm, and a certain distance should be maintained between the test benches, cabinets and other equipment for easy cleaning.

The laboratory door is a purification door. There should be a window and an automatic door closer. The doors in the laboratory must be opened in place. And to achieve an appropriate fire rating, the door lock and door opening direction should not hinder the escape of indoor personnel.

The laboratory should be equipped with a sink, and the faucet switch should be non-manual located near the exit.

The water supply pipeline should be equipped with backflow preventers or other effective devices; the water supply and drainage system should be free of leakage with anti-backflow design.

Appropriate emergency equipment is required, such as fire fighting equipment, accident handling equipment, first aid equipment, etc.
Appropriate communication devices.

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