High-speed refrigerated centrifuges are conventional laboratory centrifuges, which are widely used in biology, chemistry, medicine, and other scientific research, education, and production departments; due to the high speed, it is recommended that when users buy a centrifuge, except the centrifuge speed, centrifugal force, and rotor capacity. Besides, for meeting the basic experimental needs, special attention should be paid to the safety performance and performance of the centrifuge such as the centrifuge, which is equipped with automatic rotor recognition function, the rotor has biological safety protection function, fast cooling function, explosion-proof safety protection function, etc., To ensure the laboratory personnel in a safe experimental environment, this article will focus on the related introduction.
High-speed refrigerated centrifuges generally have a maximum speed of 10,000 to 30000 RPM, and the separation form is solid-liquid sedimentation separation, generally equipped with various angle rotors, horizontal rotors, zone rotors, vertical rotors, etc.; It has refrigeration system to eliminate the heat generated by the friction between the rotor and the air during high-speed rotation. The temperature of the centrifugal chamber can be adjusted and maintained at 0-40°C. Speed, temperature, and time can be strictly and accurately controlled and displayed, usually used for the separation and purification of microbial cells, cell debris, large organelles, ammonium sulfate precipitates, and immunoprecipitates.
At present, high-speed refrigerated centrifuges have relatively strict requirements on the main shaft, motor, rotor, refrigeration, safety performance, etc., so the high-speed refrigerated centrifuge brands frequently selected by users on the market are relatively concentrated, such as Eppendorf, Heraeus, Sigma, Heal Force, etc.
So, how to choose a high-speed refrigerated centrifuge with highly comprehensive cost performance? My suggestions are as follows.
Purchasing Recommendations the Centrifuge
1. The speed, centrifugal force, and rotor capacity should meet the experimental requirements or expansion requirements.
The most basic requirements for choosing a high-speed refrigerated centrifuge are that the rotation speed and centrifugal force must not be lower than the actual requirements required for the experiment, and the centrifuge rotor must meet the needs of various specifications of centrifuge tubes required for the experiment. If conditions permit, it is recommended to purchase a high-speed refrigerated centrifuge that is slightly larger or larger than the specifications required for the current experiment to meet future experimental expansion needs and avoid purchasing again.
The core of centrifugal separation is centrifugal force. One of the main factors for the successful separation of experimental samples is centrifugal force; due to different centrifugal radii and other factors, there will be a large difference in centrifugal force at the same speed. The maximum speed of the centrifuge is 23300RPM, but the centrifugal force actually exceeds 50000g, so this centrifuge can basically meet the experimental conditions required for routine scientific research experiments. There are many desktop high-speed refrigerated centrifuges with a speed of 25000RPM or higher. The maximum centrifugal force can only reach about 35000g.
2.Outstanding centrifugal performance:
The main performances of high-speed refrigerated centrifuges include time for speeding up and down, cooling speed, the versatility of centrifuge, humanized operation, etc. Some of them will be selected for a brief introduction;
Time for speeding up and down: This performance is particularly concerned by users in micro desktop high-speed centrifuges, such as the micro-speed and high-speed centrifuge commonly used in molecular biology. Speed up to 12000RPM, the time required by different brands varies from 12 seconds to 25 seconds, for example, Heal Force Neofuge13R centrifuge takes about 13 seconds (Note: Some experiments require natural speed reduction or slower speed, the better, such as white membrane layer separation);
Refrigeration speed: General molecular biology experiments require centrifugation at 4°C. Since the centrifuge is placed in the laboratory, the temperature of the chamber of the centrifuge must be stabilized at about 4°C before the sample is placed in the centrifuge to prevent the sample Denaturation and other damages, so it is recommended to choose a centrifuge with pre-cooling function and rapid cooling to reduce the waiting time and provide a stable temperature environment, for example, Heal Force Neofuge13R centrifuge from room temperature 25 ℃ to 4 ℃ only takes 10 minutes.
Centrifuge versatility: a laboratory may carry out different experiments at the same time. Sometimes, it not only needs 1.5ml centrifuge tubes, but also centrifuge tubes with different specifications such as 50ml, 15ml, and even larger capacity, such as 400ml centrifuge bottles. At this time, it is recommended to buy a desktop high-speed universal centrifuge to meet all the above centrifugal needs, such as the Heal Force Neofuge1600R centrifuge.
3. Security protection performance
When choosing a centrifuge, in addition to paying attention to the speed, centrifugal force, capacity, and performance, because the rotor of the high-speed centrifuge is running at a very high speed, in case the centrifuge accidentally breaks the shaft or the rotor breaks unexpectedly, It is very important whether the centrifuge can effectively ensure the personal safety of the experiment operators and avoid the occurrence of experimental accidents.
In terms of the safety design of high-speed refrigerated centrifuges, it is recommended that you choose a centrifuge with an automatic rotor recognition function to avoid possible safety accidents caused by incorrect operation of the experiment operator when the centrifugal speed value is set to exceed the maximum speed specified by the rotor.
If the experiment requires centrifugal separation of samples with pathogenic microorganisms, in order to avoid experimental operators being exposed to possible bioaerosols, it is recommended to choose an air-tight centrifugal rotor with biological safety protection function in a biological safety cabinet The rotor is opened to protect the operator, and at the same time, the rotor can be sterilized at high temperature and humidity.
The high-speed refrigerated centrifuge is home to routine laboratory equipment. Due to its high frequency of use, it is particularly important to choose a centrifuge with outstanding performance, high safety protection, and beautiful appearance.
It is recommended that when purchasing a centrifuge, in addition to the speed, centrifugal force, rotor capacity and lifting speed meeting the experimental requirements, special attention should be paid to the safety performance and performance of the centrifuge, such as rotor automatic identification function, rotor biological safety protection function, rapid cooling Function, explosion-proof safety protection function, etc., to ensure that the experimenters work in a safe experimental environment, and at the same time ensure the safety of the experimental samples.