TaqMan probe
The TaqMan probe is a fluorescent probe that specifically binds to the template. Its 5’end is labeled with a fluorescent reporter (Reporter, R), and its 3’end is labeled with a fluorescent quencher (Quencher, Q). When the probe is intact, the fluorophore and the quenching group are very close, so that the fluorescence emitted by the fluorophore is quenched. As PCR progresses, Taq enzyme encounters the probe bound to the template during the chain extension process, and its 5′-3′ exonuclease activity will cleave the probe, and the released 5’end reporter group will be free in the reaction system. , It is not affected by the 3’end fluorescence quenching group, the fluorescence signal can be detected by the instrument, and the fluorescence is accumulated. It can also be said that every time a DNA strand is amplified, a fluorescent molecule is formed, and the accumulation of fluorescent signal is synchronized with the formation of PCR products. The technology is currently widely used, including human or animal pathogen detection, biological product identification and other fields.
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