What is African swine fever？
African swine fever (ASF) is an acute, hemorrhagic and severe infectious disease caused by the infection of domestic swine and various wild boars (African wild boar, European wild boar, etc.) by the African swine fever virus (ASFV). The World Organisation for Animal Health (Original name in French: Office International des Épizooties, OIE) has listed it as a statutory reportable animal disease, which is global priority prevention.
ASFV enters the pig’s body through the mouth and upper respiratory tract system. Infection occurs in the nasopharynx or tonsils. The virus quickly spreads to the mandibular lymph nodes and spreads throughout the body through the lymph and blood.
The cells change rapidly during strong virus infection, and the cells have died before showing a significant stimulus-response; during weak virus infection, the stimulus-response is easily observed, the nucleus becomes larger, and mitosis generally occurs.
The morbidity is usually between 40% and 85%, and the mortality varies depending on the infected strain. The mortality of highly pathogenic strains can reach 90-100%; The mortality of moderate pathogenic strains in adult animals is between 20% and 40%, and in young animals, it is between 70% and 80%; The mortality of low pathogenic strains is between 10% and 30%.
Application of PCR in ASF detection
According to the report on African Swine Fever issued by OIE, real-time PCR can be used to detect and analyze the swine fever virus.
In order to strengthen the prevention and control of African swine fever and help various testing institutions to quickly establish an African swine fever (ASF) detection system, Heal Force has provided the “Heal Force African swine fever (ASF) detection overall solution”. It integrates the convenient collection, rapid extraction, and integration of samples, and cooperates with diverse, safe and reliable laboratory testing equipment and inspection management systems to help you quickly build a laboratory testing system that complies with ASF testing standards.
▲ In addition to the application of PCR in the detection of African swine fever, PCR can also detect the recent outbreak of coronavirus (COVID-2019). Heal Force also has one related solution.
The equipment that may be used in this solution is as follows:
The T960 Touch cycler is the flagship of the Heal Force thermal cycling platform, offering unmatched performance for fast, accurate results and art interface with new ways to optimize protocols and meet any researcher’s unique needs.
The X960 real-time PCR system is a high-performance benchtop instrument giving you greater control of your experiment data. It delivers reliability, sensitivity, and accuracy, which is optimized to enable the broadest range of quantitative PCR applications.
With an extensive track record of safety, reliability, and performance, HFsafe LC cabinets make ideal investments for a wide range of applications including work with infectious agents that require Biosafety Level 1, 2 or 3 containment.
The Smart N system is a compact solution that combines the production of pure water and ultrapure water in a single unit — eliminating the need for a pretreatment stage upstream of the ultrapure water system.
The 13R is Heal Force’s high-speed refrigerated centrifuge is suitable for molecular biology and testing laboratories. The product has obtained CFDA medical record production and has a high-cost performance.
Heal Force biosafety cabinets and laminar flow clean benches set the standard in quality, design, and innovation that comes from a heritage of over 25 years’ experience.
Nucleic Acid Extraction Instruments
Please contact us to get the latest lab equipment solution
If we have these devices, we can start to extract and detect the African swine fever virus, and we will provide you with the detection methods for the subsequent extraction.